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Santa Cruz Biotechnology
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Image Search Results
Journal: EMBO Reports
Article Title: Notch‐inducing hydrogels reveal a perivascular switch of mesenchymal stem cell fate
doi: 10.15252/embr.201845964
Figure Lengend Snippet: Representative images of micro‐capillary networks by CD31‐immunostains at day 7.
Article Snippet: Antibody Application/dilution Catalog number Mouse
Techniques:
Journal: EMBO Reports
Article Title: Notch‐inducing hydrogels reveal a perivascular switch of mesenchymal stem cell fate
doi: 10.15252/embr.201845964
Figure Lengend Snippet: Antibodies
Article Snippet: Antibody Application/dilution Catalog number Mouse
Techniques:
Journal: EMBO Reports
Article Title: Notch‐inducing hydrogels reveal a perivascular switch of mesenchymal stem cell fate
doi: 10.15252/embr.201845964
Figure Lengend Snippet: Gene expression of BM‐MSCs after (7 days) culture in Jagged1‐functionalized 3D microenvironments and subsequent (7 days) exposure to new Jagged1‐functionalized or IgG control microenvironments. Gene expression was analyzed by qRT–PCR and normalized on 3 reference genes (GAPDH, YWHAZ, EEF1A1). Bars represent mean values ± SD, n = 5, ANOVA with Bonferroni's post hoc test shows significant differences from IgG baseline control (dashed line): *P < 0.05, **P < 0.01, ***P < 0.001,****P < 0.0001.Source data are available online for this figure.
Article Snippet: Antibody Application/dilution Catalog number Mouse anti‐CD31 ICC 1:150 BD Bioscience 555444 Rabbit a‐CD31 ICC 1:50 Abcam ab28364 Mouse a‐FN (IST‐9) ICC 1:300 Santa Cruz sc59826 Mouse a‐COL1 ICC 1:150 Abcam ab90395 Rabbit a‐COL3 ICC 1:150 Abcam ab7778 Mouse a‐COL4 ICC 1:150 Abcam ab6311 Mouse a‐HSPG‐2 ICC 1:200 Abcam ab23418 Rabbit a‐Laminin 1+2 ICC 1:150 Abcam ab7463 Goat a‐mouse‐488 ICC 1:200 Abcam ab150113 Goat a‐rabbit‐488 ICC 1:200 Abcam ab150077 Goat a‐rabbit‐568 ICC 1:200 Abcam ab175471 Goat a‐mouse‐555 ICC 1:200 Biolegend 405324 Mouse a‐alpha‐Tubulin WB: 1:2,000 Sigma T‐5168 Mouse a‐FN (IST4) WB 1:1,000 Sigma F‐0916 Goat a‐mouse‐HRP WB 1:2,000 DAKO P0447 Mouse a‐SMA ICC 1:100 Sigma A‐2547 Rabbit a‐PDGFR‐β ICC 1:100 Cell Signaling 3169 Rabbit a‐MCAM ICC 1:200 Abcam ab75769 Mouse a‐CD31‐PE FACS 1:50
Techniques: Expressing, Quantitative RT-PCR
Journal: EMBO Reports
Article Title: Notch‐inducing hydrogels reveal a perivascular switch of mesenchymal stem cell fate
doi: 10.15252/embr.201845964
Figure Lengend Snippet: Antibodies
Article Snippet: Antibody Application/dilution Catalog number Mouse anti‐CD31 ICC 1:150 BD Bioscience 555444 Rabbit a‐CD31 ICC 1:50 Abcam ab28364 Mouse a‐FN (IST‐9) ICC 1:300 Santa Cruz sc59826 Mouse a‐COL1 ICC 1:150 Abcam ab90395 Rabbit a‐COL3 ICC 1:150 Abcam ab7778 Mouse a‐COL4 ICC 1:150 Abcam ab6311 Mouse a‐HSPG‐2 ICC 1:200 Abcam ab23418 Rabbit a‐Laminin 1+2 ICC 1:150 Abcam ab7463 Goat a‐mouse‐488 ICC 1:200 Abcam ab150113 Goat a‐rabbit‐488 ICC 1:200 Abcam ab150077 Goat a‐rabbit‐568 ICC 1:200 Abcam ab175471 Goat a‐mouse‐555 ICC 1:200 Biolegend 405324 Mouse a‐alpha‐Tubulin WB: 1:2,000 Sigma T‐5168 Mouse a‐FN (IST4) WB 1:1,000 Sigma F‐0916 Goat a‐mouse‐HRP WB 1:2,000 DAKO P0447 Mouse a‐SMA ICC 1:100 Sigma A‐2547 Rabbit a‐PDGFR‐β ICC 1:100 Cell Signaling 3169 Rabbit a‐MCAM ICC 1:200 Abcam ab75769 Mouse a‐CD31‐PE FACS 1:50
Techniques:
Journal: EMBO Reports
Article Title: Notch‐inducing hydrogels reveal a perivascular switch of mesenchymal stem cell fate
doi: 10.15252/embr.201845964
Figure Lengend Snippet: Antibodies
Article Snippet: Antibody Application/dilution Catalog number Mouse anti‐CD31 ICC 1:150 BD Bioscience 555444 Rabbit a‐CD31 ICC 1:50 Abcam ab28364 Mouse a‐FN (IST‐9) ICC 1:300 Santa Cruz sc59826 Mouse a‐COL1 ICC 1:150 Abcam ab90395 Rabbit a‐COL3 ICC 1:150 Abcam ab7778 Mouse a‐COL4 ICC 1:150 Abcam ab6311 Mouse a‐HSPG‐2 ICC 1:200 Abcam ab23418 Rabbit a‐Laminin 1+2 ICC 1:150 Abcam ab7463 Goat a‐mouse‐488 ICC 1:200 Abcam ab150113 Goat a‐rabbit‐488 ICC 1:200 Abcam ab150077 Goat a‐rabbit‐568 ICC 1:200 Abcam ab175471 Goat a‐mouse‐555 ICC 1:200 Biolegend 405324 Mouse a‐alpha‐Tubulin WB: 1:2,000 Sigma T‐5168 Mouse a‐FN (IST4) WB 1:1,000 Sigma F‐0916 Goat a‐mouse‐HRP WB 1:2,000 DAKO P0447 Mouse a‐SMA ICC 1:100 Sigma A‐2547 Rabbit a‐PDGFR‐β ICC 1:100
Techniques:
Journal: Diabetes
Article Title: Cardioprotective and anti-inflammatory effects of interleukin converting enzyme inhibition in experimental diabetic cardiomyopathy.
doi: 10.2337/db06-1662
Figure Lengend Snippet: FIG. 3. Representative histological pictures of CD68 and CD11b cells (magnification 200 and 1,000, marked by black arrows) of an STZ animal. CD11b cell with 1,000 magnification demonstrating an intravascular leukocyte before undergoing transendothelial migration. Bars representing data from histological evaluation of immunocompetent cells positive for the macrophage marker CD68 and for the leukocyte markers CD18, CD11a, and CD11b in cardiac tissue of control, STZ-induced diabetes, and STZ treated with the ICEI (STZICEI). *Significantly different compared with control and STZICEI, with P < 0.05. §Significantly different compared with control and STZ.
Article Snippet: Staining was performed with the following primary antibodies at the dilutions given (45 min, room temperature): for mouse anti-rat: ICAM-1 (clone 1A29, 1:100; Serotec, Munich, Germany), VCAM-1 (clone 5F10, 1:50; BabCO, Richmond, VA), CD18 (clone WT3, 1:50; Serotec), CD11a (clone WT1, 1:100; Serotec), CD11b (clone MRC OX-42, 1:100; Biozol, Eching, Germany), COL 1 (1:200; Abcam Limited, Cambridge, U.K.), Col3 (clone FH-7A, 1:40.000; Abcam Limited), and
Techniques: Migration, Marker, Control