col 3 (α1) 488-goat anti-rabbit dapi Search Results


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Becton Dickinson mouse anti cd31 icc 1:150
Representative images of micro‐capillary networks by <t>CD31‐immunostains</t> at day 7.
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Becton Dickinson mouse igg1, k isotype control pe facs 1:50
Gene expression of BM‐MSCs after (7 days) culture in Jagged1‐functionalized 3D microenvironments and subsequent (7 days) exposure to new Jagged1‐functionalized or <t>IgG</t> control microenvironments. Gene expression was analyzed by qRT–PCR and normalized on 3 reference genes (GAPDH, YWHAZ, EEF1A1). Bars represent mean values ± SD, n = 5, ANOVA with Bonferroni's post hoc test shows significant differences from IgG baseline control (dashed line): *P < 0.05, **P < 0.01, ***P < 0.001,****P < 0.0001.Source data are available online for this figure.
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Gene expression of BM‐MSCs after (7 days) culture in Jagged1‐functionalized 3D microenvironments and subsequent (7 days) exposure to new Jagged1‐functionalized or <t>IgG</t> control microenvironments. Gene expression was analyzed by qRT–PCR and normalized on 3 reference genes (GAPDH, YWHAZ, EEF1A1). Bars represent mean values ± SD, n = 5, ANOVA with Bonferroni's post hoc test shows significant differences from IgG baseline control (dashed line): *P < 0.05, **P < 0.01, ***P < 0.001,****P < 0.0001.Source data are available online for this figure.
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Cell Signaling Technology Inc 3169 rabbit
Antibodies
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Bio-Rad cd68
FIG. 3. Representative histological pictures of <t>CD68</t> and CD11b cells (magnification 200 and 1,000, marked by black arrows) of an STZ animal. CD11b cell with 1,000 magnification demonstrating an intravascular leukocyte before undergoing transendothelial migration. Bars representing data from histological evaluation of immunocompetent cells positive for the macrophage marker CD68 and for the leukocyte markers CD18, CD11a, and CD11b in cardiac tissue of control, STZ-induced diabetes, and STZ treated with the ICEI (STZICEI). *Significantly different compared with control and STZICEI, with P < 0.05. §Significantly different compared with control and STZ.
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Cell Signaling Technology Inc cell signaling 3180
FIG. 3. Representative histological pictures of <t>CD68</t> and CD11b cells (magnification 200 and 1,000, marked by black arrows) of an STZ animal. CD11b cell with 1,000 magnification demonstrating an intravascular leukocyte before undergoing transendothelial migration. Bars representing data from histological evaluation of immunocompetent cells positive for the macrophage marker CD68 and for the leukocyte markers CD18, CD11a, and CD11b in cardiac tissue of control, STZ-induced diabetes, and STZ treated with the ICEI (STZICEI). *Significantly different compared with control and STZICEI, with P < 0.05. §Significantly different compared with control and STZ.
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Cell Signaling Technology Inc cell signaling 2217
FIG. 3. Representative histological pictures of <t>CD68</t> and CD11b cells (magnification 200 and 1,000, marked by black arrows) of an STZ animal. CD11b cell with 1,000 magnification demonstrating an intravascular leukocyte before undergoing transendothelial migration. Bars representing data from histological evaluation of immunocompetent cells positive for the macrophage marker CD68 and for the leukocyte markers CD18, CD11a, and CD11b in cardiac tissue of control, STZ-induced diabetes, and STZ treated with the ICEI (STZICEI). *Significantly different compared with control and STZICEI, with P < 0.05. §Significantly different compared with control and STZ.
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Cell Signaling Technology Inc cell signaling 2535
FIG. 3. Representative histological pictures of <t>CD68</t> and CD11b cells (magnification 200 and 1,000, marked by black arrows) of an STZ animal. CD11b cell with 1,000 magnification demonstrating an intravascular leukocyte before undergoing transendothelial migration. Bars representing data from histological evaluation of immunocompetent cells positive for the macrophage marker CD68 and for the leukocyte markers CD18, CD11a, and CD11b in cardiac tissue of control, STZ-induced diabetes, and STZ treated with the ICEI (STZICEI). *Significantly different compared with control and STZICEI, with P < 0.05. §Significantly different compared with control and STZ.
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Cell Signaling Technology Inc cell signaling 2118
FIG. 3. Representative histological pictures of <t>CD68</t> and CD11b cells (magnification 200 and 1,000, marked by black arrows) of an STZ animal. CD11b cell with 1,000 magnification demonstrating an intravascular leukocyte before undergoing transendothelial migration. Bars representing data from histological evaluation of immunocompetent cells positive for the macrophage marker CD68 and for the leukocyte markers CD18, CD11a, and CD11b in cardiac tissue of control, STZ-induced diabetes, and STZ treated with the ICEI (STZICEI). *Significantly different compared with control and STZICEI, with P < 0.05. §Significantly different compared with control and STZ.
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Cell Signaling Technology Inc cell signaling 3033
FIG. 3. Representative histological pictures of <t>CD68</t> and CD11b cells (magnification 200 and 1,000, marked by black arrows) of an STZ animal. CD11b cell with 1,000 magnification demonstrating an intravascular leukocyte before undergoing transendothelial migration. Bars representing data from histological evaluation of immunocompetent cells positive for the macrophage marker CD68 and for the leukocyte markers CD18, CD11a, and CD11b in cardiac tissue of control, STZ-induced diabetes, and STZ treated with the ICEI (STZICEI). *Significantly different compared with control and STZICEI, with P < 0.05. §Significantly different compared with control and STZ.
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Santa Cruz Biotechnology santa cruz sc
FIG. 3. Representative histological pictures of <t>CD68</t> and CD11b cells (magnification 200 and 1,000, marked by black arrows) of an STZ animal. CD11b cell with 1,000 magnification demonstrating an intravascular leukocyte before undergoing transendothelial migration. Bars representing data from histological evaluation of immunocompetent cells positive for the macrophage marker CD68 and for the leukocyte markers CD18, CD11a, and CD11b in cardiac tissue of control, STZ-induced diabetes, and STZ treated with the ICEI (STZICEI). *Significantly different compared with control and STZICEI, with P < 0.05. §Significantly different compared with control and STZ.
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Image Search Results


Representative images of micro‐capillary networks by CD31‐immunostains at day 7.

Journal: EMBO Reports

Article Title: Notch‐inducing hydrogels reveal a perivascular switch of mesenchymal stem cell fate

doi: 10.15252/embr.201845964

Figure Lengend Snippet: Representative images of micro‐capillary networks by CD31‐immunostains at day 7.

Article Snippet: Antibody Application/dilution Catalog number Mouse anti‐CD31 ICC 1:150 BD Bioscience 555444 Rabbit a‐CD31 ICC 1:50 Abcam ab28364 Mouse a‐FN (IST‐9) ICC 1:300 Santa Cruz sc59826 Mouse a‐COL1 ICC 1:150 Abcam ab90395 Rabbit a‐COL3 ICC 1:150 Abcam ab7778 Mouse a‐COL4 ICC 1:150 Abcam ab6311 Mouse a‐HSPG‐2 ICC 1:200 Abcam ab23418 Rabbit a‐Laminin 1+2 ICC 1:150 Abcam ab7463 Goat a‐mouse‐488 ICC 1:200 Abcam ab150113 Goat a‐rabbit‐488 ICC 1:200 Abcam ab150077 Goat a‐rabbit‐568 ICC 1:200 Abcam ab175471 Goat a‐mouse‐555 ICC 1:200 Biolegend 405324 Mouse a‐alpha‐Tubulin WB: 1:2,000 Sigma T‐5168 Mouse a‐FN (IST4) WB 1:1,000 Sigma F‐0916 Goat a‐mouse‐HRP WB 1:2,000 DAKO P0447 Mouse a‐SMA ICC 1:100 Sigma A‐2547 Rabbit a‐PDGFR‐β ICC 1:100 Cell Signaling 3169 Rabbit a‐MCAM ICC 1:200 Abcam ab75769 Mouse a‐CD31‐PE FACS 1:50 BD Bioscience 560983 Mouse IgG1, k isotype control‐PE FACS 1:50 BD Bioscience 556650 Open in a separate window Antibodies

Techniques:

Antibodies

Journal: EMBO Reports

Article Title: Notch‐inducing hydrogels reveal a perivascular switch of mesenchymal stem cell fate

doi: 10.15252/embr.201845964

Figure Lengend Snippet: Antibodies

Article Snippet: Antibody Application/dilution Catalog number Mouse anti‐CD31 ICC 1:150 BD Bioscience 555444 Rabbit a‐CD31 ICC 1:50 Abcam ab28364 Mouse a‐FN (IST‐9) ICC 1:300 Santa Cruz sc59826 Mouse a‐COL1 ICC 1:150 Abcam ab90395 Rabbit a‐COL3 ICC 1:150 Abcam ab7778 Mouse a‐COL4 ICC 1:150 Abcam ab6311 Mouse a‐HSPG‐2 ICC 1:200 Abcam ab23418 Rabbit a‐Laminin 1+2 ICC 1:150 Abcam ab7463 Goat a‐mouse‐488 ICC 1:200 Abcam ab150113 Goat a‐rabbit‐488 ICC 1:200 Abcam ab150077 Goat a‐rabbit‐568 ICC 1:200 Abcam ab175471 Goat a‐mouse‐555 ICC 1:200 Biolegend 405324 Mouse a‐alpha‐Tubulin WB: 1:2,000 Sigma T‐5168 Mouse a‐FN (IST4) WB 1:1,000 Sigma F‐0916 Goat a‐mouse‐HRP WB 1:2,000 DAKO P0447 Mouse a‐SMA ICC 1:100 Sigma A‐2547 Rabbit a‐PDGFR‐β ICC 1:100 Cell Signaling 3169 Rabbit a‐MCAM ICC 1:200 Abcam ab75769 Mouse a‐CD31‐PE FACS 1:50 BD Bioscience 560983 Mouse IgG1, k isotype control‐PE FACS 1:50 BD Bioscience 556650 Open in a separate window Antibodies

Techniques:

Gene expression of BM‐MSCs after (7 days) culture in Jagged1‐functionalized 3D microenvironments and subsequent (7 days) exposure to new Jagged1‐functionalized or IgG control microenvironments. Gene expression was analyzed by qRT–PCR and normalized on 3 reference genes (GAPDH, YWHAZ, EEF1A1). Bars represent mean values ± SD, n = 5, ANOVA with Bonferroni's post hoc test shows significant differences from IgG baseline control (dashed line): *P < 0.05, **P < 0.01, ***P < 0.001,****P < 0.0001.Source data are available online for this figure.

Journal: EMBO Reports

Article Title: Notch‐inducing hydrogels reveal a perivascular switch of mesenchymal stem cell fate

doi: 10.15252/embr.201845964

Figure Lengend Snippet: Gene expression of BM‐MSCs after (7 days) culture in Jagged1‐functionalized 3D microenvironments and subsequent (7 days) exposure to new Jagged1‐functionalized or IgG control microenvironments. Gene expression was analyzed by qRT–PCR and normalized on 3 reference genes (GAPDH, YWHAZ, EEF1A1). Bars represent mean values ± SD, n = 5, ANOVA with Bonferroni's post hoc test shows significant differences from IgG baseline control (dashed line): *P < 0.05, **P < 0.01, ***P < 0.001,****P < 0.0001.Source data are available online for this figure.

Article Snippet: Antibody Application/dilution Catalog number Mouse anti‐CD31 ICC 1:150 BD Bioscience 555444 Rabbit a‐CD31 ICC 1:50 Abcam ab28364 Mouse a‐FN (IST‐9) ICC 1:300 Santa Cruz sc59826 Mouse a‐COL1 ICC 1:150 Abcam ab90395 Rabbit a‐COL3 ICC 1:150 Abcam ab7778 Mouse a‐COL4 ICC 1:150 Abcam ab6311 Mouse a‐HSPG‐2 ICC 1:200 Abcam ab23418 Rabbit a‐Laminin 1+2 ICC 1:150 Abcam ab7463 Goat a‐mouse‐488 ICC 1:200 Abcam ab150113 Goat a‐rabbit‐488 ICC 1:200 Abcam ab150077 Goat a‐rabbit‐568 ICC 1:200 Abcam ab175471 Goat a‐mouse‐555 ICC 1:200 Biolegend 405324 Mouse a‐alpha‐Tubulin WB: 1:2,000 Sigma T‐5168 Mouse a‐FN (IST4) WB 1:1,000 Sigma F‐0916 Goat a‐mouse‐HRP WB 1:2,000 DAKO P0447 Mouse a‐SMA ICC 1:100 Sigma A‐2547 Rabbit a‐PDGFR‐β ICC 1:100 Cell Signaling 3169 Rabbit a‐MCAM ICC 1:200 Abcam ab75769 Mouse a‐CD31‐PE FACS 1:50 BD Bioscience 560983 Mouse IgG1, k isotype control‐PE FACS 1:50 BD Bioscience 556650 Open in a separate window Antibodies

Techniques: Expressing, Quantitative RT-PCR

Antibodies

Journal: EMBO Reports

Article Title: Notch‐inducing hydrogels reveal a perivascular switch of mesenchymal stem cell fate

doi: 10.15252/embr.201845964

Figure Lengend Snippet: Antibodies

Article Snippet: Antibody Application/dilution Catalog number Mouse anti‐CD31 ICC 1:150 BD Bioscience 555444 Rabbit a‐CD31 ICC 1:50 Abcam ab28364 Mouse a‐FN (IST‐9) ICC 1:300 Santa Cruz sc59826 Mouse a‐COL1 ICC 1:150 Abcam ab90395 Rabbit a‐COL3 ICC 1:150 Abcam ab7778 Mouse a‐COL4 ICC 1:150 Abcam ab6311 Mouse a‐HSPG‐2 ICC 1:200 Abcam ab23418 Rabbit a‐Laminin 1+2 ICC 1:150 Abcam ab7463 Goat a‐mouse‐488 ICC 1:200 Abcam ab150113 Goat a‐rabbit‐488 ICC 1:200 Abcam ab150077 Goat a‐rabbit‐568 ICC 1:200 Abcam ab175471 Goat a‐mouse‐555 ICC 1:200 Biolegend 405324 Mouse a‐alpha‐Tubulin WB: 1:2,000 Sigma T‐5168 Mouse a‐FN (IST4) WB 1:1,000 Sigma F‐0916 Goat a‐mouse‐HRP WB 1:2,000 DAKO P0447 Mouse a‐SMA ICC 1:100 Sigma A‐2547 Rabbit a‐PDGFR‐β ICC 1:100 Cell Signaling 3169 Rabbit a‐MCAM ICC 1:200 Abcam ab75769 Mouse a‐CD31‐PE FACS 1:50 BD Bioscience 560983 Mouse IgG1, k isotype control‐PE FACS 1:50 BD Bioscience 556650 Open in a separate window Antibodies

Techniques:

Antibodies

Journal: EMBO Reports

Article Title: Notch‐inducing hydrogels reveal a perivascular switch of mesenchymal stem cell fate

doi: 10.15252/embr.201845964

Figure Lengend Snippet: Antibodies

Article Snippet: Antibody Application/dilution Catalog number Mouse anti‐CD31 ICC 1:150 BD Bioscience 555444 Rabbit a‐CD31 ICC 1:50 Abcam ab28364 Mouse a‐FN (IST‐9) ICC 1:300 Santa Cruz sc59826 Mouse a‐COL1 ICC 1:150 Abcam ab90395 Rabbit a‐COL3 ICC 1:150 Abcam ab7778 Mouse a‐COL4 ICC 1:150 Abcam ab6311 Mouse a‐HSPG‐2 ICC 1:200 Abcam ab23418 Rabbit a‐Laminin 1+2 ICC 1:150 Abcam ab7463 Goat a‐mouse‐488 ICC 1:200 Abcam ab150113 Goat a‐rabbit‐488 ICC 1:200 Abcam ab150077 Goat a‐rabbit‐568 ICC 1:200 Abcam ab175471 Goat a‐mouse‐555 ICC 1:200 Biolegend 405324 Mouse a‐alpha‐Tubulin WB: 1:2,000 Sigma T‐5168 Mouse a‐FN (IST4) WB 1:1,000 Sigma F‐0916 Goat a‐mouse‐HRP WB 1:2,000 DAKO P0447 Mouse a‐SMA ICC 1:100 Sigma A‐2547 Rabbit a‐PDGFR‐β ICC 1:100 Cell Signaling 3169 Rabbit a‐MCAM ICC 1:200 Abcam ab75769 Mouse a‐CD31‐PE FACS 1:50 BD Bioscience 560983 Mouse IgG1, k isotype control‐PE FACS 1:50 BD Bioscience 556650 Open in a separate window Antibodies

Techniques:

FIG. 3. Representative histological pictures of CD68 and CD11b cells (magnification 200 and 1,000, marked by black arrows) of an STZ animal. CD11b cell with 1,000 magnification demonstrating an intravascular leukocyte before undergoing transendothelial migration. Bars representing data from histological evaluation of immunocompetent cells positive for the macrophage marker CD68 and for the leukocyte markers CD18, CD11a, and CD11b in cardiac tissue of control, STZ-induced diabetes, and STZ treated with the ICEI (STZICEI). *Significantly different compared with control and STZICEI, with P < 0.05. §Significantly different compared with control and STZ.

Journal: Diabetes

Article Title: Cardioprotective and anti-inflammatory effects of interleukin converting enzyme inhibition in experimental diabetic cardiomyopathy.

doi: 10.2337/db06-1662

Figure Lengend Snippet: FIG. 3. Representative histological pictures of CD68 and CD11b cells (magnification 200 and 1,000, marked by black arrows) of an STZ animal. CD11b cell with 1,000 magnification demonstrating an intravascular leukocyte before undergoing transendothelial migration. Bars representing data from histological evaluation of immunocompetent cells positive for the macrophage marker CD68 and for the leukocyte markers CD18, CD11a, and CD11b in cardiac tissue of control, STZ-induced diabetes, and STZ treated with the ICEI (STZICEI). *Significantly different compared with control and STZICEI, with P < 0.05. §Significantly different compared with control and STZ.

Article Snippet: Staining was performed with the following primary antibodies at the dilutions given (45 min, room temperature): for mouse anti-rat: ICAM-1 (clone 1A29, 1:100; Serotec, Munich, Germany), VCAM-1 (clone 5F10, 1:50; BabCO, Richmond, VA), CD18 (clone WT3, 1:50; Serotec), CD11a (clone WT1, 1:100; Serotec), CD11b (clone MRC OX-42, 1:100; Biozol, Eching, Germany), COL 1 (1:200; Abcam Limited, Cambridge, U.K.), Col3 (clone FH-7A, 1:40.000; Abcam Limited), and CD68 (clone ED1, 1:2,000; Serotec); for goat anti-rat: TNF- (1:25; R&D Systems, Wiesbaden, Germany) and IL1- (1:25; R&D Systems); and for rabbit anti-rat: IL-18 (1:25; R&D Systems).

Techniques: Migration, Marker, Control